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Construction and Application of A Marker Localizing in Nucleolus and Cajal Body
Zheng Luping1,2, Mao Qianzhuo1, Lin Chen1, Xie Liyan1, Wu Zujian1*, Xie Lianhui1*
1Key Laboratory of Plant Viology , Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry Univer
Abstract: cDNA encoding Nicotiana benthamiana fibrillarin2 was amplified by RT-PCR and inserted into expressed vectors which include YFP or CFP. Transient expression of these constructs was on leaf epidermal cells of Nicotiana benthamiana， these proteins localized in the nucleolus and cajal body， which can be a marker of these two organelles. The expression of these new fusion proteins were also verified by Western blot. This marker maybe play a role on indicating accurate localization in nucleus and forecasting the function of proteins， especially virus-encoded proteins.
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