Experimental Study of Transplantation of Rabbit Endothelial Progenitor Cells Transfected by Ad-BMP-2-IRES-HIF-1αmu for Treatment of Avascularnecrosis of the Femoral Head

Abstract: The induced EPCs were transfected by Ad-BMP-2-IRES-HIF-1αmu， and then transplanted into femoral head necrotic zone， the effect on osteogenesis and agiogenesis of necrosis zone was detected. MNCs were obtained by density gradient centrifugation method， and were induced into EPCs by M199 medium; EPCs were identified through cell morphology， specific surface markers and uptake ability. EPCs were transfected by Ad-BMP-2-IRES-HIF-1αmu， and then were transplanted into partial of ANFH. The model were enthanized at 2 and 4 weeks after operation， effection on osteogenesis and agiogenesis of necrotic zone was detected. The results showed that there were more agiogenesis in group A than that in group B and C (P<0.05)， the statistical difference was found between group B and C. The detection of histology and immunohistochemistry of BMP-2 showed that there was statistical difference between group A and B， group A and C (P<0.05). There was no statistical difference between group B and C (P>0.05). To sum up， there was an effection on osteogenesis and agiogenesis in EPCs after transfeced by Ad-BMP-2-IRES-HIF-1αmu.

CSTR: 32200.14.cjcb.2013.07.0010