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An Analysis on the Transcriptional Activity of Nanog Pluripotent Transcription Factor in Lung Adenocarcinoma Stem Cells
Huang Jinsu1, Zhang Yan2, Li Rong1, Wu Lixia2, Ning Renli2, Xu Huili2, Dong Qianggang2*
1Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiaotong University Medical School, Shanghai 200030, China; 2Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University Medical School, Shanghai 200032
Abstract: An integrated analysis of shRNA-mediated gene knockdown and cDNA-based microarray was performed to screen out the genes expressed differentially after inactivation of Nanog pluripotent gene in lung adenocarcinoma stem cells (CSC). The results showed that Nanog inactivation elicited 1 605 genes that were expressed differentially， among which 95 genes belong to the known Nanog downstream target genes. With KEGG analysis， a critical function of Nanog was found to repress the expression of genes encoding for the components of proteasome. Moreover， in the unique gene signature of these CSCs， 25 genes were shown to be regulated by Nanog. Its inactivation elicited the up-regulation of 15 genes and the down-regulation of 10 genes. These data indicated that Nanog pluripotent factor is functional in the gene transcription in lung adenocarcinoma stem cells.
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