The Effect of Chinese Medicine (Compound) on SOD， CAT Activities and Bax Protein， TGF-β1 Expression of Filial Mice Kidney Injured by Ephedrine

Abstract: To explore protection of Chinese medicine against stimulant injury， the ephedrine group intraperitoneal injected 0.2 mL escalation doses (2.0， 3.0， 4.0 g/L) of ephedrine， and Chinese medicine group intragastric with 0.2 mL the concentration of 20.0， 30.0， 40.0 g/L of Chinese medicine (compound) after injected ephedrine 1 h respectively. At 5， 10， 15 d， the activities of SOD， CAT as well as content of MDA were detected by colorimetry respectively. In addition， the expression of Bax protein and TGF-β1 were measured by immunohistochemistry in kidney of mice at the different developmental stages. Results showed the content of MDA in ephedrine group increased (P<0.05， P<0.01); activity of SOD and CAT decreased (P<0.05， P<0.01); the expression of Bax protein and TGF-β1 increased in kidney. The content of MDA in Chinese medicine group decreased (P<0.05， P<0.01); activity of SOD and CAT increased (P<0.05， P<0.01); the expression of Bax protein and TGF-β1 decreased in kidney. The Chinese medicine (compound) could improve the cell anti-oxidantion， inhibit cell lipd peroxidation and the expression of Bax and TGF-β1， promote the organization cell repair and reduce cell damage by improving cell activity and inhibiting apoptosis. The Chinese medicine (compound) helps to protect and cue ephedrine-caused tissue damage.

CSTR: 32200.14.cjcb.2013.07.0007