Home > Browse Issues > Vol.31 No.4
Stamen Development and Gene Expression of Flower of Male-induced Bitter Melon Gynoecious Line
Ri-Sheng Wang 1,2,3, Yang-Rui Li3*, Sheng-Mao Zhou 2, Man Zhang 2, Feng-Xue Fang 2, Li-Tao Yang 1
1Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning 530005, China; 2Guangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Sciences, Nanning 5
Abstract: To elucidate the differential gene expression during the development of stamens in female flower buds of bitter melon gynoecious line induced with AgNO3， in the present study， it was studied at the levels of morphology and transcription genomics using morphologic observations， cDNA-AFLP， RT-PCR and reverse-Northern hybridization. The results showed as follows: (1) After treated with AgNO3， stamens were generated at the 6th day， and bisexual flowers with viable pollen grains were observed at the 14th day， and gynoecious flowers were recovered since the 22th day. (2) Thirty-six primer combinations could amplify many distinct bands， among them， 11 bands which were strongly expressed only in the AgNO3-treated buds were cloned， sequenced and aligned on line， resulting in discovery of 2 unknown genes and 9 known genes. The sequence showing high similarity with CYP450 gene super family which related to plant sex differentiation was named as McCYP72A1. (3) McCYP72A1 was expressed in the flower buds during the period of 2-12 h after treated with AgNO3， and expressed most strongly at 8 h， but not expressed in the roots， stems， leaves of treatment and the mixed samples of buds， roots， stems， leaves of the control. It suggested that genes related to male differentiation in the flower buds of bitter melon gynoecious line were expressed after treated with AgNO3， which might make female flowers develop into bisexual flowers within 14 days.
CN
EN