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Synergistic Inhibition Effect of Onconase and Sodium Cantharidate on the Growth of Human Lung Adenocarcinoma Cells
Zhai Changbao1, Shen Ruling2, Hu Xiaojun1, Xu Diansheng1, Fei Jian2,3, Wang Qingcheng2*
1East China University of Science and Technology, Shanghai 200237, China; 2Shanghai Research Center For Model Organisms,Shanghai 201203, China; 3Tongji University-Guizhou Shenqi Research Center of Ethnomedicine, Shanghai 2
Abstract: Onconase (Onc) is a ribonuclease purified from oocytes of Rana pipiens， which possesses potent antitumor activity. Cantharidin is a natural defensive toxin， existing in Mylabrisphalerata pallas or Mylabriscichorii linnaeus. Sodium cantharidate is a semi-synthetic derivative of cantharidin. The cytotoxicity of Onconase and sodium cantharidate alone or combined on the growth of SPC-A-1 and A549 non-small cell lung cancer (NSCLC) cell lines was measured by MTT method. The anti-tumor joint effect of Onconase and sodium cantharidate in vitro was assessed by combination indices and isobologram analyses. The experimental results show that the combination indices were less than 0.7， and the points of joint effect were located below the line of additivity. The combination of Onconase and sodium cantharidate exhibited a synergistic growth-inhibitory effect on both SPC-A-1 and A549 cell lines. The data of apoptotic cells studied by flow cytometry were consistent with above conclusion. The Onc/SCA combination may have potential in NSCLC therapy.
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