Generation of Chitinase Domain Containing 1 (Chid1) Knockout Mice

Abstract: To generate chitinase domain containing 1 (Chid1) knockout mice， mice were observed in phenotype and development. We designed an appropriate strategy and constructed the targeting vector. After electroporation of targeting vector into ES cells， positive clones were grown and selected using G418 and Ganciclovir， then injected into mouse blastulas to obtain chimeric mice. By mating with C57BL/6J mice， we got heterozygous mice with Aguoti coat. Then we got homozygous KO mice from heterozygousmice mating. RT-PCR results showed that Chid1 expressed in brain， spleen， liver and lung from heterozygous and wild-type mice， but not in homozygous mice. In general， Chid1 knockout mice develope normally and all mice are alive after birth， there is no defect in fertility and development， Chid1 knockout mice are successfully generated and can be a good animal model for investigate its biological function in vivo.

CSTR: 32200.14.cjcb.2013.03.0009