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MicroRNA-10b Blocks the Differentiation of Leukemia Cells Through Regulating the Zinc Finger Protein KLF4
Tan Shi, Zhang Huijuan, Wang Juan, Chen Shana, Quan Jing, Xian Jingrong, Zhang Ling*
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Faculty of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
Abstract: We aimed to explore the effect of microRNA-10b (miR-10b) on the differentiation of leukemia cells through regulating the expression of zinc finger protein Krüppel-like factor 4 (KLF4). The expression of miR-10b and KLF4 in leukemia cell lines at different levels of differentiation was detected by Real-time PCR and Western blot， respectively. Leukemia cell line HL60 was induced with 1，25-dihydroxy-vitamin D3 (1，25D3) to differentiate along the monocytic lineage. The expression of miR-10b and KLF4 was examined during 1，25D3-induced differentiation of HL60. The synthesized miR-10b mimics was transfected into HL60 cells. The morphological changes of cells treated with 1，25D3 were observed under light microscope following Wright-Giemsa staining， and the monocyte surface marker CD14 was analyzed by flow cytometry. miR-10b was detected at the highest levels of expression in KG-1a cells displaying early differentiation phenotypes and at the lowest in more mature U937 and THP-1 cells (P<0.01)， while the KLF4 exhibited an opposite expression pattern. miR-10b was decreased in a time-dependent manner in HL60 cells during induction with 1，25D3， whereas the KLF4 was increased. Enforced expression of miR-10b in HL60 cells inhibited the 1，25D3-induced morphological changes and the expression of CD14 (P<0.05). Our data indicate that miR-10b suppresses monocytic differentiation of HL60 cells via targeting to KLF4.
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