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Research of Inducing Mouse Embryonic Stem Cells Differentiate into Skeletal Muscle Cells in vitro
Ye Feng, Tong Huili, Du Wei, Yan Yunqin*
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China
Abstract: Mouse embryonic stem cells are separated from the inner cell mass of embryo， with the abilities of unlimited growth， self-renewal and differentiation potential when cultured in vitro. Mouse embryonic stem cells can be induced to differentiate into muscle cells， which can be used in treatment of muscle degenerative diseases and is becoming a research focus of the field. The aim of this study was to obtain the effective conditions of inducing mouse embryonic stem cells differentiate into skeletal muscle cells. Results showed that 10-8 mol/L retinoid acid (RA) and 0.5% dimethyl sulfoxide (DMSO) could induce mouse embryonic stem cells differentiate into skeletal muscle precursor cells with the highest efficiency， and the skeletal muscle precursor cells purified to differentiate into multinucleated myotubes. It provided cells for the treatment of muscle degenerative diseases and also can be beneficial to the study of the mechanism about mouse embryonic stem cells differentiate into skeletal muscle.
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