Home > Browse Issues > Vol.35 No.2
The Effect of Aquaglyceroporin 7 on Protein Kinase B Signaling Pathway
Pan Wei1, Shen Feixia1*, Gu Xuemei1, Ye Jing1, Gu Xuejiang1, Ni Liansong1, Li Weiping2
11Department of Endocrinology and Metabolism, First Affilliated Hospital, Wenzhou Medical College, Wenzhou 325000, China;22Department of Endocrinology and Metabolism, First Affiliated Hospital, Medical College, Shantou University,Sha
Abstract: Aquaglyceroporin 7 (AQP7) is a water transporting protein which also regulates the glycerol
efflux in adipocytes. The study of AQP7 might shed new light on the prevention and control of obesity. In this
study， we aimed to analyze expression profiles of AQP7 in the different differentiation phase of adipocytes and the
relationship between AQP7 and PKB in the insulin pathway. 3T3-L1 preadipocyte cells were induced fully differentiated.
Insulin resistance in differentiated adipocytes was induced by TNF-α. Adenovirus overexpression AQP7
(Ad-AQP7) was constructed and transfected to adipocytes. The expression levels of AQP7 and phosphorylated
PKB (p-PKB) were measured. The glycerol release from adipocytes and the change of glucose concentration in the
culture medium were also tested.The AQP7 levels were gradually up-regulated along with the differentiation phase
of 3T3-L1 preadipocytes， which was consistent with the expression levels of p-PKB. Overexpression of AQP7 by
transfecting Ad-AQP7 to insulin resistant adipocytes could improve insulin sensitivity and glucose utilization， in
accroding with the level of p-PKB expression. The expression of AQP7 correlated with phosphorylation of PKB.
Overexpression of AQP7 contributed to improve insulin resistance in adipocytes.
efflux in adipocytes. The study of AQP7 might shed new light on the prevention and control of obesity. In this
study， we aimed to analyze expression profiles of AQP7 in the different differentiation phase of adipocytes and the
relationship between AQP7 and PKB in the insulin pathway. 3T3-L1 preadipocyte cells were induced fully differentiated.
Insulin resistance in differentiated adipocytes was induced by TNF-α. Adenovirus overexpression AQP7
(Ad-AQP7) was constructed and transfected to adipocytes. The expression levels of AQP7 and phosphorylated
PKB (p-PKB) were measured. The glycerol release from adipocytes and the change of glucose concentration in the
culture medium were also tested.The AQP7 levels were gradually up-regulated along with the differentiation phase
of 3T3-L1 preadipocytes， which was consistent with the expression levels of p-PKB. Overexpression of AQP7 by
transfecting Ad-AQP7 to insulin resistant adipocytes could improve insulin sensitivity and glucose utilization， in
accroding with the level of p-PKB expression. The expression of AQP7 correlated with phosphorylation of PKB.
Overexpression of AQP7 contributed to improve insulin resistance in adipocytes.
CN
EN