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Analysis of Gene Expression Profile Changes Responding to siRNAmediated PRR11 Depletion in Lung Cancer H1299 Cells
Long Yinjiang1,2, Ji Ying1,2, Weng Huali1,2, Zhang Chundong1,2, Xie Mengyu1,2, Cai Wei1,2,Wang Yitao1,2, Zhu Yuanyuan1,2, Li Yi1,2, Zhang Ying1,2*
1Department of Biochemistry and Molecular Biology, ChongQing Medical University, Chongqing 400016, China;2Molecular Medicine and Cancer Research Center, ChongQing Medical University, Chongqing 400016, China
Abstract: Proline rich 11 (PRR11) is a novel cancer-associated gene identified by our group. In the present
study， in order to clarifying the molecular mechanisms of PRR11 in lung cancer development， the gene expression
profile changes responding to the siRNA-mediated PRR11 depletion was analyzed. siRNAs were used to inhibit
the PRR11 expression， total RNAs were then prepared and subjected to microarray analysis. The differentially
expressed genes were enriched for GO and pathway analysis. qRT-PCR was used to verify several important
differentially expressed genes. Microarray analysis revealed that siRNA-mediated PRR11 depletion resulted into
the expression changes of 550 genes including 139 upregulated and 411 downregulated. Bioinformatic analysis
indicated that the 550 differentially expressed genes were mainly enriched in cell cycle and MAPK pathways. qRTPCR analysis verified that siRNA-mediated PRR11 depletion led to the expression changes of several cell cycleand
cancer-related genes including DHRS2， EPB41L3， CCNA1， MAP4K4， RRM1 and NFIB. Taken together，
the present study suggested that PRR11 might be implicated in lung cancer development via regulating the
aforementioned genes and/or pathways.
study， in order to clarifying the molecular mechanisms of PRR11 in lung cancer development， the gene expression
profile changes responding to the siRNA-mediated PRR11 depletion was analyzed. siRNAs were used to inhibit
the PRR11 expression， total RNAs were then prepared and subjected to microarray analysis. The differentially
expressed genes were enriched for GO and pathway analysis. qRT-PCR was used to verify several important
differentially expressed genes. Microarray analysis revealed that siRNA-mediated PRR11 depletion resulted into
the expression changes of 550 genes including 139 upregulated and 411 downregulated. Bioinformatic analysis
indicated that the 550 differentially expressed genes were mainly enriched in cell cycle and MAPK pathways. qRTPCR analysis verified that siRNA-mediated PRR11 depletion led to the expression changes of several cell cycleand
cancer-related genes including DHRS2， EPB41L3， CCNA1， MAP4K4， RRM1 and NFIB. Taken together，
the present study suggested that PRR11 might be implicated in lung cancer development via regulating the
aforementioned genes and/or pathways.
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