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The Influence of the Apoptosis Rate and the Expression of Apoptosis Proteins in the Macrophages Infected by Mycobacterium tuberculosis
Dong Weijie, Li Wei, Liu Danxia, Liu Yunxia, Tuo Qingzhang, Wu Fang, Zhang Le, Zhang Wanjiang*
Department of Microbiology Immunology, Medical School, Shihezi University; Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi University, Shihezi 832002, China
Abstract: To explore the regulation and mechanism of apoptosis of the macrophage infected by Mycobacterium
tuberculosis. Infected the macrophage RAW264.7 cell line with international standards with Mycobacterium
tuberculosis H37Rv and BCG， at the same time， set the blank control group， after the infection at the 1， 6， 12， 24 h，
flow cytometry were employed to detect the rate of the apoptosis of macrophages of each group. Then detect the
Caspase-3 protein and expression levels of the gene with Western blot. The results showed that the rate of the apoptosis
of macrophage RAW264.7 cell line infected by Mycobacterium tuberculosis is significantly higher than that of the control group， the difference was statistically significant (P<0.05)， and the rate of apoptosis of macrophage
RAW264.7 cell line infected by the BCG was higher than that of the H37Rv， after infection， at 1， 12， 24 h， the rate
of the apoptosis increased significantly， and the difference was statistically significant (P<0.05). After being infected
by Mycobacterium tuberculosis， the expression of the Caspase-3 protein in macrophages increased， and the
expression of the Caspase-3 protein of the Mycobacterium tuberculosis group is higher than the control group， the
control group The expression of Caspase3 protein in BCG infection group is higher than that in H37Rv infection group， at 1， 12，
24 h， the expression of protein was significantly increased after being infected， the difference was statistically significant
(P<0.05). The expression level of Caspase-3 gene in the Mycobacterium tuberculosis infection group was
relatively higher than that in the control group， the difference was statistically significant (P<0.05). The expression
of the Bcl-2 protein of the Mycobacterium tuberculosis infection group was significantly lower than that of the
control group， the difference was statistically significant (P<0.05). At 1， 6， 24 h， the expression of Bcl-2 protein in
the H37Rv infection group was higher than that in the BCG infection group after being infected， the difference was
statistically significant (P<0.05). This shows the infection with Mycobacterium tuberculosis leads to the apoptosis
of macrophages， the intensity of virulence of Mycobacterium tuberculosis was related to the apoptosis of cells. And
the apoptosis of macrophages infected with Mycobacterium tuberculosis was related to the expression of Caspase-3
and Bcl-2.
tuberculosis. Infected the macrophage RAW264.7 cell line with international standards with Mycobacterium
tuberculosis H37Rv and BCG， at the same time， set the blank control group， after the infection at the 1， 6， 12， 24 h，
flow cytometry were employed to detect the rate of the apoptosis of macrophages of each group. Then detect the
Caspase-3 protein and expression levels of the gene with Western blot. The results showed that the rate of the apoptosis
of macrophage RAW264.7 cell line infected by Mycobacterium tuberculosis is significantly higher than that of the control group， the difference was statistically significant (P<0.05)， and the rate of apoptosis of macrophage
RAW264.7 cell line infected by the BCG was higher than that of the H37Rv， after infection， at 1， 12， 24 h， the rate
of the apoptosis increased significantly， and the difference was statistically significant (P<0.05). After being infected
by Mycobacterium tuberculosis， the expression of the Caspase-3 protein in macrophages increased， and the
expression of the Caspase-3 protein of the Mycobacterium tuberculosis group is higher than the control group， the
control group
24 h， the expression of protein was significantly increased after being infected， the difference was statistically significant
(P<0.05). The expression level of Caspase-3 gene in the Mycobacterium tuberculosis infection group was
relatively higher than that in the control group， the difference was statistically significant (P<0.05). The expression
of the Bcl-2 protein of the Mycobacterium tuberculosis infection group was significantly lower than that of the
control group， the difference was statistically significant (P<0.05). At 1， 6， 24 h， the expression of Bcl-2 protein in
the H37Rv infection group was higher than that in the BCG infection group after being infected， the difference was
statistically significant (P<0.05). This shows the infection with Mycobacterium tuberculosis leads to the apoptosis
of macrophages， the intensity of virulence of Mycobacterium tuberculosis was related to the apoptosis of cells. And
the apoptosis of macrophages infected with Mycobacterium tuberculosis was related to the expression of Caspase-3
and Bcl-2.
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