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The Effect of Tumor Microenvironment to Morphology， Growth and Proliferation of Human Mesenchymal Stem Cells-bone Marrow
Liu Yongqi1,2,3*, Wang Qian1, Qin Jie1, Li Yi1,4, She Yali1,2,3, Li Jingya1
1Institute of Systems Biology and TCM Transformation, Gansu Traditional Chinese Medicine College, Lanzhou 730000,China;
2Basis Room of Integration of Traditional and Western Medicine, Key Laboratory of Traditional Chinese Medicine
2Basis Room of Integration of Traditional and Western Medicine, Key Laboratory of Traditional Chinese Medicine
Abstract: Because of the advantages of human mesenchymal stem cells-bone marrow (HMSC-bm) such
as easy to be isolated， cultured， expanded in vitro and imported exogenous gene， the tropism to tumor， the lowdown
immunogenicity etc.， it becomes an ideal target therapeutic vector for tumor’s biological treatment in recent
years. In this study， a co-culture system of HMSC-bm and lung adenocarcinoma cell line A549 was established by
using Transwell chamber. To study the related biological characteristics’ changes of HMSC-bm， induced for 3 and
7 days， and then， passaged to the third generation and the fifth generation respectively in tumor microenvironment.
The results showed that along with the extension of co-culture time and the increase of the passage， morphology of
the experimental groups cells changed gradually. MTT assay and Cell cycle analysis indicated that the experimental groups cells growth vigor was reinforced gradually. The results suggested HMSC-bm was induced to differentiate
in lung adenocarcinoma microenvironment， its biological characteristics such as morphology， growth and proliferation
were changed. The HMSC-bm may be has tumorigenicity. But the mechanism of differentiation should be
studied deeply to provide the scientific evidence for widely clinical application of HMSC-bm.
as easy to be isolated， cultured， expanded in vitro and imported exogenous gene， the tropism to tumor， the lowdown
immunogenicity etc.， it becomes an ideal target therapeutic vector for tumor’s biological treatment in recent
years. In this study， a co-culture system of HMSC-bm and lung adenocarcinoma cell line A549 was established by
using Transwell chamber. To study the related biological characteristics’ changes of HMSC-bm， induced for 3 and
7 days， and then， passaged to the third generation and the fifth generation respectively in tumor microenvironment.
The results showed that along with the extension of co-culture time and the increase of the passage， morphology of
the experimental groups cells changed gradually. MTT assay and Cell cycle analysis indicated that the experimental groups cells growth vigor was reinforced gradually. The results suggested HMSC-bm was induced to differentiate
in lung adenocarcinoma microenvironment， its biological characteristics such as morphology， growth and proliferation
were changed. The HMSC-bm may be has tumorigenicity. But the mechanism of differentiation should be
studied deeply to provide the scientific evidence for widely clinical application of HMSC-bm.
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