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ST6Gal-I Regulates Human Choriocarcinoma JAR Cells Migration via Up-regulating α2，6-sialylation of Integrin β1
Zhu Ying1, Yu Chao2, Liu Xueqing1, Chen Xuemei1, Ding Yubin1, Wang Yingxiong1,2, He Junlin1*
1Laboratory of Reproductive Biology, School of Public Health, Chongqing 400016, China;
2Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
2Institute of Life Science, Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the effect of β-galactoside α2，6-sialyltransferase-1 (ST6Gal-I) on migration of
human choriocarcinoma cells (JAR) and human ectravillous trophoblast cells (HTR-8/SVneo)， we first compared
the migration ability between JAR cells and HTR-8/SVneo cells via Transwell chamber. Then the expression levels
of ST6Gal-I mRNA and protein in both cells was detected by real-time PCR and Western blot. Finally， ST6Gal-I
protein of JAR cells was down-regulated by siRNA， and then the cell migration was measured by transwell chamber，
at the same time the sialylation of integrin β1 which is the target protein of ST6Gal-I was detected by immunoprecipitation.
The results showed that the migration of JAR cells was stronger than HTR-8/SVneo cells， consistently
levels of ST6Gal-I mRNA and protein in JAR cells were much higher than HTR-8/SVneo cells. Interference of
ST6Gal-I mRNA in JAR cells reduced sialylation of integrin β1， and at last inhibited cell migration. These studies have shown that ST6Gal-I was involved in the regulation of tumor cell migration， and it was the important revelation
for finding new therapeutic targets.
human choriocarcinoma cells (JAR) and human ectravillous trophoblast cells (HTR-8/SVneo)， we first compared
the migration ability between JAR cells and HTR-8/SVneo cells via Transwell chamber. Then the expression levels
of ST6Gal-I mRNA and protein in both cells was detected by real-time PCR and Western blot. Finally， ST6Gal-I
protein of JAR cells was down-regulated by siRNA， and then the cell migration was measured by transwell chamber，
at the same time the sialylation of integrin β1 which is the target protein of ST6Gal-I was detected by immunoprecipitation.
The results showed that the migration of JAR cells was stronger than HTR-8/SVneo cells， consistently
levels of ST6Gal-I mRNA and protein in JAR cells were much higher than HTR-8/SVneo cells. Interference of
ST6Gal-I mRNA in JAR cells reduced sialylation of integrin β1， and at last inhibited cell migration. These studies have shown that ST6Gal-I was involved in the regulation of tumor cell migration， and it was the important revelation
for finding new therapeutic targets.
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