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Dictyostelium discoideum Cell Adhesion Molecule cadA/DdCAD-1 Plays An Important Role in Cell Differentiation and Cell Fate Determination
Yang Chunxia, Hou Liansheng*
(School of Life Science, East China Normal University, Shanghai 200062, China)
Abstract: Cell adhesion molecule cadA/DdCAD-1 is the first expressed adhesion molecule during the development
of Dictyostelium discoideum， to investigate the role of DdCAD-1 in D. discoideum development， cadA
mutant strain cadA– cells were colored by Natural Red， slug structure show obviously aberrant cell differentiation.
Exogenous recombinant protein His6-DdCAD-1 rescued this phenomenon. Additionally， recombinant protein can
also rescue the decreasing spore yield of cadA– cells. When DdCAD-1 expressed cells were mixed with cadA– cells，
DdCAD-1 expressed cells occupied the tips and posterior region of the chimeric culmination structures， which do
not belong to spore area and would die eventually. All these results suggest that DdCAD-1 plays a crucial role in
cell differentiation and cell fate determination.
of Dictyostelium discoideum， to investigate the role of DdCAD-1 in D. discoideum development， cadA
mutant strain cadA– cells were colored by Natural Red， slug structure show obviously aberrant cell differentiation.
Exogenous recombinant protein His6-DdCAD-1 rescued this phenomenon. Additionally， recombinant protein can
also rescue the decreasing spore yield of cadA– cells. When DdCAD-1 expressed cells were mixed with cadA– cells，
DdCAD-1 expressed cells occupied the tips and posterior region of the chimeric culmination structures， which do
not belong to spore area and would die eventually. All these results suggest that DdCAD-1 plays a crucial role in
cell differentiation and cell fate determination.
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