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The Effect and Mechanism of Lipoxin A4 Suppressing Scar Formation
Zhou Xiaoyan1*, Kuang Xiaodong2, Huang Yonghong1, Xu Fangyun1, Cai Zhenyu1, Wang Hongmei1*
1Medical College of Nanchang University Department of Pathophysiology, Nanchang 330006, China;
2Medical College of Nanchang University Department of Pathology, Nanchang 330006, China
2Medical College of Nanchang University Department of Pathology, Nanchang 330006, China
Abstract: It was reported that Lipoxin A4 (LXA4) could prevent and cure tissue fibrosis and related disease.
In this paper， we observed the effects of LXA4 on the proliferation， apoptosis and collagen synthesis of keloid
fibroblast， and analyzed the possible mechanisms. Firstly， we tested the expression of ALX through RT-PCR and
Western blot. Then， we examined the proliferation by MTT， the apoptosis through flow cytometry， the degree of
collagen synthesis with hydroxyproline testing cassete， and the level of TGF-β via ELISA. The data demonstrated
that ALX was expressed in hyperplastic scar fibroblast cell， LXA4 inhibited the proliferation and collagen synthesis，
LXA4 induced cell apoptosis and LXA4 decreased the level of TGF-β. It seemed LXA4 was an important potential
drug for prevention and control of scar formation.
In this paper， we observed the effects of LXA4 on the proliferation， apoptosis and collagen synthesis of keloid
fibroblast， and analyzed the possible mechanisms. Firstly， we tested the expression of ALX through RT-PCR and
Western blot. Then， we examined the proliferation by MTT， the apoptosis through flow cytometry， the degree of
collagen synthesis with hydroxyproline testing cassete， and the level of TGF-β via ELISA. The data demonstrated
that ALX was expressed in hyperplastic scar fibroblast cell， LXA4 inhibited the proliferation and collagen synthesis，
LXA4 induced cell apoptosis and LXA4 decreased the level of TGF-β. It seemed LXA4 was an important potential
drug for prevention and control of scar formation.
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