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Study on the Cell Toxicity of the Lectin-like Domain of RvvhA
Xu Ling, Xie Danli, Zhang Liuli, Lou Yongliang*
School of Medical Lab Science, Wenzhou Medical College, Wenzhou 325035, China
Abstract: VVC amino acid sequence 313-445 (Gi No. M34670) is highly homologous to the B chain of
ricin. We obtained the purified rRicin B proteins by gene cloning， purification， renaturation and purification. Rabbit
polyclonal antibody against rRicin B was prepared by immunning rabbit and was purified by persulfate and Sephadex
G25 followed by analysis of Western blot hybridization and antibody titer. Observation of the effect of rRicin
B on cell apoptosis and rabbit polyclonal antibody (Endnotes) protection by flow cytometry， we found that rabbit
polyclonal antibody against rRicin B was purified successfully by methods of Persulfate and Sephadex G25. Polyclonal
antibody showed high immuno-reactivity. The results of flow cytometry observation indicated that rRicin B
can inhibit the growth of Hela cells and the effect was depended on doses of rRicin B. Polyclonal antibody could
block up the damage of Hela cells acted by rRicin B. All the results showed that rabbit polyclonal antibody against
rRicin B was prepared successfully with cell toxicity， but the cell toxicity of rRicin B could be blocked by its polyclonal
antibodies.
ricin. We obtained the purified rRicin B proteins by gene cloning， purification， renaturation and purification. Rabbit
polyclonal antibody against rRicin B was prepared by immunning rabbit and was purified by persulfate and Sephadex
G25 followed by analysis of Western blot hybridization and antibody titer. Observation of the effect of rRicin
B on cell apoptosis and rabbit polyclonal antibody (Endnotes) protection by flow cytometry， we found that rabbit
polyclonal antibody against rRicin B was purified successfully by methods of Persulfate and Sephadex G25. Polyclonal
antibody showed high immuno-reactivity. The results of flow cytometry observation indicated that rRicin B
can inhibit the growth of Hela cells and the effect was depended on doses of rRicin B. Polyclonal antibody could
block up the damage of Hela cells acted by rRicin B. All the results showed that rabbit polyclonal antibody against
rRicin B was prepared successfully with cell toxicity， but the cell toxicity of rRicin B could be blocked by its polyclonal
antibodies.
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