Home > Browse Issues > Vol.35 No.1
Study the Function of GRP78 in Cell Proliferation Process in Esophageal Cancer Cell Line ECA-109
Ding Weichao1,2,3, Ma Yanyan 1,2, Qiu Xianxiu1,2, Wang Shaoxiang1,2, Wang Ying3*, Ren Zhe1,2*
1Biomedicine Research and Development Center of Jinan University, Guangdong Provincial Key Laboratory of Bioengineering Medicine, Guangzhou 510632, China;
2National Engineering Research Center of Genetic Medicine, Guangzhou 51063
2National Engineering Research Center of Genetic Medicine, Guangzhou 51063
Abstract: In China， esophageal cancer is a malignant tumor of high incidence and high mortality. Sustained
proliferation of tumor cells is closely related to the cell proliferation disorder. Large amounts of proteins need
to be synthesized during tumor cell proliferation process. GRP78 as a molecular chaperone， plays an important role
in the process of protein folding， assembly， modification and misfolded protein degradation. The research studied
the influence of proliferation ability with over-expression or knock-down of GRP78 in ECA-109 cells through transient
transfection of GRP78 recombinant plasmid pGRP78-EGFP-N1 or siRNA， respectively. The study found that
after GRP78 over-expression， more cells converted from G1 phage to S and G2/M phages， then cell proliferation rate
and cell clony formation rate were increased; as well as after knock-down GRP78， more cells were arrested in G1
phage and couldn’t transfer into S and G2/M phages， then cell proliferation rate and cell clony formation rate were reduced. GRP78 may promote ECA-109 cell proliferation by regulating the cell cycle.
proliferation of tumor cells is closely related to the cell proliferation disorder. Large amounts of proteins need
to be synthesized during tumor cell proliferation process. GRP78 as a molecular chaperone， plays an important role
in the process of protein folding， assembly， modification and misfolded protein degradation. The research studied
the influence of proliferation ability with over-expression or knock-down of GRP78 in ECA-109 cells through transient
transfection of GRP78 recombinant plasmid pGRP78-EGFP-N1 or siRNA， respectively. The study found that
after GRP78 over-expression， more cells converted from G1 phage to S and G2/M phages， then cell proliferation rate
and cell clony formation rate were increased; as well as after knock-down GRP78， more cells were arrested in G1
phage and couldn’t transfer into S and G2/M phages， then cell proliferation rate and cell clony formation rate were reduced. GRP78 may promote ECA-109 cell proliferation by regulating the cell cycle.
CN
EN