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Preliminary Screening of Differentially Methylated Genes in Androgenindependent
Qin Tingting1, Xu Gang1, Ji Lili1, Tao Zhihua1,2*
1Department of Clinical Laboratory Medicine, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China;
2Department of Laboratory Medicine Second Affiliated Hospital, Zhejiang University School of Medicine, Hangz
2Department of Laboratory Medicine Second Affiliated Hospital, Zhejiang University School of Medicine, Hangz
Abstract: An integrated analysis of Illumina Infinium HumanMethylation27 BeadChip platform and
transcriptome RNA sequencing technology was performed to screen out the differential methylated genes between
androgen-dependent prostate cancer cell lines and androgen-independent prostate cancer cell lines. Compared
with LNCaP， 990 hypermethylated CpG sites (involved 855 genes) and 2 305 hypomethylated CpG sites (involved
1 970 genes) were identified in LNCaP-AI. Combined with transcriptome sequencing data， some hypermethylated
genes (including FAM111B， RAB36， PCDH7， COL6A2， IGF1R and GULP1) and hypomethylated genes (including
EPHA3， TM4SF1， IGFBP5， FAM105A， RASD1， ITPR2， CYP27B1 and UBE2E3) were screened out. These differential
methylated genes were thought to be involved in some signaling pathways， such as the calcium signaling
pathway and the Wnt signaling pathway， participating in cell biological process and its regulation， gene expression，signal transduction， cell communication， cell movement， cell adhesion， angiogenesis and so on. Our data provide a
foundation for future study on the differential methylated genes in prostate cancer androgen-independent conversion
process.
transcriptome RNA sequencing technology was performed to screen out the differential methylated genes between
androgen-dependent prostate cancer cell lines and androgen-independent prostate cancer cell lines. Compared
with LNCaP， 990 hypermethylated CpG sites (involved 855 genes) and 2 305 hypomethylated CpG sites (involved
1 970 genes) were identified in LNCaP-AI. Combined with transcriptome sequencing data， some hypermethylated
genes (including FAM111B， RAB36， PCDH7， COL6A2， IGF1R and GULP1) and hypomethylated genes (including
EPHA3， TM4SF1， IGFBP5， FAM105A， RASD1， ITPR2， CYP27B1 and UBE2E3) were screened out. These differential
methylated genes were thought to be involved in some signaling pathways， such as the calcium signaling
pathway and the Wnt signaling pathway， participating in cell biological process and its regulation， gene expression，signal transduction， cell communication， cell movement， cell adhesion， angiogenesis and so on. Our data provide a
foundation for future study on the differential methylated genes in prostate cancer androgen-independent conversion
process.
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