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The Effect of Kikyosaponin on Expression of IL-1β and TNF-α from
He Lili, Chen Qin*, Peng Shenming, Cao Yangui, Li Yang, Zhu Min
School of Life Science, Anhui University, Anhui Province Key Laboratory of Research and Development of Chinese Medicine,Hefei 230039, China
Abstract: To research the expression of inflammatory cytokines of pneumonocyte of chronic bronchitis
(CB) mice and study the mechanism of kikyosaponin on bronchitis treatment， fifty healthy mice were divided into
five groups: normal control group， CB model group and treatment groups with low， middle and high doses of kikyosaponin
groups. Each group of mice inhaled dense smoke and stronger ammonia water to establish a model of
chronic bronchitis except the normal control group， and then respectively were treated by kikyosaponin. After the
experiment， the lung tissues of mice from each group were removed for paraffin section. Pathology of bronchus and alveolar cells which stained by HE were observed under the light microscope. The expression of IL-1β and TNF-α
of alveolar cells was analyzed by immunohistochemical method and Western blot. Immunohistochemical method
showed that the expression of IL-1β and TNF-α in chronic bronchitis model group increased significantly compared
with the normal group. After treatment for 30 days， the expression of IL-1β and TNF-α decreased significantly in
each treated group compared with the model group. Western blot showed that the expression of IL-1β and TNF-α in
the model group increased significantly compared with the normal group. After treatment for 30 days， the expression
of IL-1β and TNF-α decreased obviously in each treated group with dose-effect relationship. Kikyosaponin can
obviously inhibit the expression of IL-1β and TNF-α in mice lung tissue. We speculate that kikyosaponin plays an
important role in anti-inflammation， suppressing cough and relieving asthma through inhibition of the generation of
inflammatory cytokines and free radical.
(CB) mice and study the mechanism of kikyosaponin on bronchitis treatment， fifty healthy mice were divided into
five groups: normal control group， CB model group and treatment groups with low， middle and high doses of kikyosaponin
groups. Each group of mice inhaled dense smoke and stronger ammonia water to establish a model of
chronic bronchitis except the normal control group， and then respectively were treated by kikyosaponin. After the
experiment， the lung tissues of mice from each group were removed for paraffin section. Pathology of bronchus and alveolar cells which stained by HE were observed under the light microscope. The expression of IL-1β and TNF-α
of alveolar cells was analyzed by immunohistochemical method and Western blot. Immunohistochemical method
showed that the expression of IL-1β and TNF-α in chronic bronchitis model group increased significantly compared
with the normal group. After treatment for 30 days， the expression of IL-1β and TNF-α decreased significantly in
each treated group compared with the model group. Western blot showed that the expression of IL-1β and TNF-α in
the model group increased significantly compared with the normal group. After treatment for 30 days， the expression
of IL-1β and TNF-α decreased obviously in each treated group with dose-effect relationship. Kikyosaponin can
obviously inhibit the expression of IL-1β and TNF-α in mice lung tissue. We speculate that kikyosaponin plays an
important role in anti-inflammation， suppressing cough and relieving asthma through inhibition of the generation of
inflammatory cytokines and free radical.
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