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Cloning and Expression Analysis of Flavonoid 3"，5"-hydroxylase in Strelitzia reginae Banks
Huang Minling1,2,3*, Fan Ronghui1,2,3
1Institute of Crop Sciences, Fujian Academy of Agricultural Science, Fuzhou 350013, China;2Flowers Research Center, Fujian Academy of Agricultural Science, Fuzhou 350013, China;2Flowers Research Center, Fujian Academy of Agricultural
Abstract: The SrF3"5"H gene cDNA sequence involved in flavonoid biosynthesis was cloned from the blue petals of Strelitzia reginae Banks using RT-PCR and RACE techniques. The cDNA sequence consists of 1 766 bp with an intact open reading frame of 1 509 bp， encoding a polypeptide of 503 amino acids. Homology analysis showed that the deduced SrF3"5"H protein was highly homologous to other F3"5"H proteins from different plants. Phylogenetic analysis indicated that SrF3"5"H was more related to F3"5"H of Saintpaulia hybrid cultivar. The semi-quantitative PCR analysis indicated that SrF3'5'H showed the highest transcript abundance in early flowering season and the transcript was abundant in the blue petals but little in yellow sepals.
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