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Krüppel-like Factor 5 Promotes VSMC Proliferation and Migration
Liu Yu1,2, Wang Haijun3, Li Aiying2, Li Nan3, Han Mei1*
1The Key Laboratory of Medical Biotechnology of Hebei, Department of Biochemistry, Institute of Basic Medicine, Hebei Medical Unibersity, Shijiazhuang 050017, China; 2Department of Biochemistry, Traditional Chinese Medical College, H
Abstract: Krüppel-like factor 5 (KLF5)， a member of the Sp/KLF family of zinc finger factors， is a key regulator of cardiovascular remodeling. To determine the role of KLF5 in cell proliferation and migration， VSMCs were infected with Ad-KLF5 to over-express KLF5， and transfected KLF5-specific siRNA to knockdown the endogenous KLF5， respectively. The activity of the cell proliferation and migration was detected using MTT assay， flow cytometric analysis， immunocytochemistry and wound healing assays， respectively. The results showed that the over-expression of KLF5 increased the expression of PCNA protein in VSMCs， and resulted in an increase in proliferation and number of S-phase cells， compared with empty vector controls. In addition， the migration activity increased in KLF5-overexpressed VSMC. Conversely， the knockdown of KLF5 by specific siRNA abolished Ang II-induced cell growth via reduction of the number of S-phase cells， inhibited the migration of VSMCs. These findings indicate that KLF5 is required for VSMC proliferation and migration.
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