Home > Browse Issues > Vol.30 No.2
The Localizational and Expressive Alteration of Nucleophosmin during the Differentiation of Human Osteosarcoma MG-63 cells Induced by the Combination of Ginsenoside Rg1
Qi-Fu Li*, Song-Lin Shi, Qing-Rong Liu, Jian Tang, Ying Liang, Jian-Ye Song
The Key Laboratory of Ministry of Education for Cell Biology & Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
Abstract: To explore the existence， distribution of nucleophosmin (NPM) in the nuclear matrix of the osteosarcoma MG-63 cells and the co-localizational relationship between NPM and the products of some interrelated genes， the MG-63 cells treated with or without the combination of ginsenoside Rg1， cinnamic acid and tanshinone II A (RCT) were selectively extracted and subjected to proteomic methodologies and laser scanning confocal microscopy (LSCM). 2-D PAGE， MS and Western blotting methods confirmed the existence of NPM and its decreased expression in the nuclear matrix proteins. LSCM observation showed that there were altered co-localization after RCT treatment between NPM and the product of genes c-fos， c-myc， RB and p53， respectively. Our study confirmed the existence of NPM in the nuclear matrix and the important role its expression and distribution played during the differentiation of MG-63 cells induced by effective ingredients from Chinese materia medica.
CN
EN