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The Apoptosis of Human Kidney Cell Induced by Ochratoxin A in Vitro
Jin-Feng Cui, Ling-Xiao Xing*, Zeng-Ning Li, Xiang-Hong Zhang, Tao Ding, Jun-Ling Wang, Xia Yan
Lab of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China
Abstract: To explore the effect of ochratoxin A (OA) on the apoptosis of human kidney cell (HKC) in vitro， HKC were treated with OA at different concentrations for 24 h and harvested for detection. The apoptosis rate of HKC was detected with flow cytometry (FCM)， the expression of caspase-3 and the level of JNK/p-JNK were determined with immunocytochemical staining and Western blot respectively. FCM analysis showed that the apoptosis rate of HKC was evaluated in OA treated group. Both caspase-3 expression and p-JNK level were significantly higher in OA treated group than that in control group or solvent control group by SP and Western blot， while the level of JNK was not changed in all the groups. OA could induce apoptosis of HKC in vitro. The activation of JNK and caspase-3 could take part in the process of OA induced HKC apoptosis.
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