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Immunofluorescence Analysis of the Distribution of KIFC1-like Protein and NUP62 during Spermiogenesis in Octopus tankahkeei
Ma Xiaoxin1, Wang Wei1, Zhu Junquan1*, Yang Wanxi2
1Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China; 2The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, China
Abstract: Immunofluorescence and confocal microscopy were employed to investigate the localization pattern of a KIFC1-like protein and NUP62 as well as their relationship during spermiogenesis in Octopus tankahkeei. In early spermatid， the nucleus was round or oval， expression of the KIFC1-like protein was not obvious， and NUP62 was abundantly located in the nuclear membrane. In intermediate spermatid， the nucleus became spindleshaped， the KIFC1-like protein was apparently expressed and located around the nucleus， and NUP62 was mainly located in the nuclear membrane. In late spermatid， the nucleus resembled a long spindle， the KIFC1-like protein was concentrated in proximity to the nuclear periphery and also located within the nucleus， and NUP62 was distributed in the nuclear membrane and within the nucleus. In immature sperm， the nucleus was cylindrical， distribution of the KIFC1-like protein and NUP62 was similar to that in late spermatid， and the KIFC1-like protein was enriched at two ends of the cell. In mature sperm， the nucleus became long rod-shaped， expression of the KIFC1-like protein was not detected， and expression of NUP62 was maintained at a high level. The results indicated that the KIFC1-like protein was probably involved in the biogenesis of acrosome and through its interaction with NUP62 or other mediator， associated with some macromolecular assembly in the nuclear membrane， thus providing the nucleus with direct shaping force or influencing the shaping process via indirect regulation.
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