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Mechanism of PDGF-BB Down-regulating SM22α Expression in Vascular Smooth Muscle Cells
Dong Lihua, Shu Yanan, Xue Zhenying, Han Mei*
Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Key Laboratory for Medical Biotechnology of Hebei Province, Key Laboratory of Neural and Vascular Biology, Ministry of Education, Hebei Medical University, Shijiazhuang 050017,
Abstract: Vascular smooth muscle cell (VSMC) phenotypic modulation is considered to be key event in the development of vascular proliferative disorders. Platelet-derived growth factor (PDGF)-BB inhibits the expression of differentiation marker genes and promotes their degradation. Here， the phosphorylation and ubiquitination of smooth muscle 22 alpha (SM22α) stimulated by PDGF-BB was identified by Western blot analysis and coimmunoprecipitation analysis. The results showed PDGF-BB induced the proliferation of VSMCs. PDGF-BB increased the expression of PCNA， and reduced the levels of SM α-actin and SM22α. PDGF-BB increased the expression of phosphorylated and ubiquitinated SM22α， and the raise of phosphorylated level was earlier than the effect to ubiquitination. Moreover， after the ERK and PKC activity were inhibited， the SM22α phosphorylation and destabilization stimulating by PDGF-BB was disappeared. Collectively， these results indicate that destabilization of SM22α following PDGF-BB is mediated， at least in part， through ERK-PKC-depended pathway.
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