Establishment and Characterization of a Cell Line Derived from Fin of Cryprinus carpiod

Abstract: A cell line， designated Koi-Fin， has been established from the fin of Cryprinus carpiod by using tissue explant techniques. Koi-Fin cell line has been continuously subcultured over 60 times and fully characterized in the aspects of morphological observation， cryopreservation， optimal growth kinetics， population doubling time and karyotyping， etc. Koi-Fin cell monolayer consists of fibroblast-like cells and its optimal growth condition is: MEM medium， 10% FBS and 25 ºC. Under such optimized condition the doubling time of Koi-Fin cell numbers is about 46.5 h. After 12 months cryopreservation in liquid nitrogen， Koi-Fin cells exhibit a viability of about (80.21±5.84)% with trypan blue staining. Chromosome typing of this cell line revealed that at its 16th passage the diploid chromosome number was 2n=100， and at the 40th passage the modal number is 52. The results of virus sensitivity test indicated that Koi-Fin cell line is susceptible to the infection of Koi Herpesvirus (KHV)， a characteristic viral-induced cytopathic effect (CPE) was observed and the viral titer (TCID50/mL) reached about 107.86±0.51. Moreover， PCR detection demonstrated that the targeted gene can be amplified by using specific primers designed targeting the viral thymidine kinase gene.

CSTR: 32200.14.cjcb.2012.08.0004