Induces Apoptosis of Acanthopanax senticosus Polysaccharides on H446 Cells

Abstract: To investigate Acanthopanax senticosus polysaccharide (ASPS) induced apoptosis in small cell lung cancer cell lines H446 and its potential mechanism. MTT assay was used to determine the inhibitory rate of ASPS with different concentrations on the proliferation of H446 cells. The cell apoptosis induced by ASPS was detected with Hoechst 33258 dye and flow cytometry (FCM). Western blot was used to detect the changes of bax， bc1-2 and p53 in protein levels in H446 cells. MTT assay showed that the proliferation of H446 cells were markedly inhibited after treatment with ASPS for 48 h; IC50 was 476.36 μg/ml; Typical morphological changes of apoptosis were observed in H446 cells with Hoechst staining after induced by ASPS. FCM analysis revealed the apoptotic rates of H446 cells treated by 0， 240， 480， 960 μg/ml ASPS were (5.02±0.4)%， (11.12±1.8)%， (19.89±2.5)%， (22.54±1.8)% respectively. Western blot results indicated that the expression of bax and p53 were increased but bcl-2 was decreased by ASPS. ASPS could inhibit the proliferation of H446 cells and induce part of cells to apoptosis. The activation of bax and p53 and the suppression of bc1-2 may contribute to the apoptosis mechanism.

CSTR: 32200.14.cjcb.2008.02.0021