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Effect of Chelation of Extracellular Calcium on Maturation of Cultured Rat Granulosa Cells
Wen Cao1, Ling-Mei Pan1,3, Yong-Qing Jiang2, Li-Zhi Lu2, Chun-Hua Meng1, Jian Li1, Kazuyoshi Taya3, Fang-Xiong Shi1*
1Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China; 3Laboratory of Veter
Abstract: Calcium is an important cellular signal. The aim of the present study is to examine the effect of chelation of extracellular calcium on the maturation of cultured rat granulosa cells. The results demonstrate that addition of EGTA， a chelator of extracellular calcium， significantly inhibits the estradiol production in cultured rat granulosa cells stimulated by FSH in a dose-dependent manner. As it is known that adenylyl cyclase VIII can be promoted by intercellular calcium， we hypothesize that the action of calcium may be through activating adenylyl cyclase VIII in rat granulosa cells. However， the results of our RT-PCR and Northern blot indicate that the role of calcium is not via adenylyl cyclase VIII isoform but may be through other pathways in cultured rat granulosa cells.
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