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The Mutation of Valine201 and Valine289 Influences the Activity of Recombiant Vibrio vulnificus Hemolysin
Guo Yajun, Ye Meiping, Wang Shuai, Lou Yongliang*
Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Mediccal College, Wenzhou 325035, China
Abstract: To compare the activity between rVvhA and rVvhAval201gly，val289gly， Val201 and Val289 were mutated to glycines and recombinant proteins were expressed after mutations. The hemolytic activity， cytotoxic， intracellular calcium influx and potassium ion release between rVvhA and rVvhAval201gly，val289gly were compared. The results showed that the hemolysis of rVvhAval201gly，val289gly was reduced. Cell death was partly rescued by blocking calcium influx and potassium ion release. In conclusion， the mutations of Val201 and Val289 are relevent with the cells ions balance， hemolysis and cytotoxicity of rVvhA.
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