Correlational Study on gp150 and Protein Kinase A During Dictyostelium discoideum Development

Abstract: When Dictyostelium discoideum entered into post-developmental stage， Dictyostelium discoideum protein kinase A (DdPKA ) activity increased significantly at 12 h， 16 h and 20 h time-points in wild-type cells of KAx-3 which was related to cell morphological differentiation. But the DdPKA activity of gp150 knocked out cells (AK127) remained at a relative high level and only decreased apparently at 22 h. However， the DdPKA activity of both stains increased at 24 h. Overall， the DdPKA activity in AK127 was higher than in KAx-3. These showed that the loss of gp150 may lead to the regulation of DdPKA activity get out of control. In addition， DdPKA activity in prestalk cells increased slowly from 16 h to 18 h but decreased rapidly at 20 h， while in prespore cells the activity rose up at 20 h after a decline at 18 h which was almost 2-fold of that in prestalk cells. The colocalization study with laser confocal microscopy showed that the positions of two DdPKA subunits were not together with each other and gp150 was much closer to DdPKA-R， and they even overlapped with each other. The results indicate that the interaction between gp150 and DdPKA-R might influences the apoptosis of prestalk cells and the differentiation of prespore cells.

CSTR: 32200.14.cjcb.2012.07.0002