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Lysophosphatidic Acid Protects Mesenchymal Stem Cells against Hypoxia and Serum Deprivation-induced Apoptosis
Lin-Hui Shi, Jing-Hai Chen, Lin-Zi Deng, Xue-Wen Liu, Xi Chen, Xiang-Feng Cong*
Research Center for Cardiovascular Regenerative Medicine, the Ministry of Health, Cardiovascular Institute and Fuwai Hospital, Peking Union Medicine College & Chinese Academy of Medical Sciences, Beijing 100037, China
Abstract: The aim of this study is to investigate the effects of lysophosphatidic acid (LPA) on the apoptosis of bone marrow-derived mesenchymal stem cells (BMMSCs). The apoptosis was detected by Hoechst33342 staining and annexin V/PI dual-color flow cytometry， and the expression of signaling proteins was evaluated by Western blot analysis. In the condition of hypoxia and serum deprivation (hypoxia/SD)， a significant apoptosis occurred in BMMSCs. The groups of LPA 6 h or LPA 1+6 h， but not LPA 1 h， significantly decreased the apoptosis induced by hypoxia/SD (P<0.05). The effect of LPA on the intracellular signal pathways was also examined. Akt was activated， while ERK1/2 was depressed by LPA under hypoxia/SD conditions. These results suggest LPA could decrease the apoptosis of BMMSCs induced by hypoxia/SD， and increase the survival of BMMSCs.
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