Cultured and Proteomic Evaluation of Resistance Powdery Mildew Near-isogenic Line Contained the H. villosum 6VS

Abstract: In this study， we cultured a near-isogenic line (NIL) of Jing 411 and we are also evaluation the selection effects of near-isogenic line by proteomic techniques. Jing 411， which is a fine wheat variety but susceptible to powdery mildew pathogen， was crossed with wheat (H. villosum) chromosome translocation line 6AL/6VS. The donor of the resistance gene， F1 individuals then were back-crossed with Jing 411 seven times and selfed to produce progenice (NIL). The proteome of leaves and roots were investigated and the results indicated that 43.8% protein spots changed had a tendency to 6AL/6VS and 56.2% had a tendency to recurrent parent Jing 411 in leaves of NILS. In roots， 41.7% protein spots changed had a tendency to 6AL/6VS and 58.3% protein spots changed tended to recurrent parent Jing 411. 23 protein spots were identified by MS/MS. They were ATP synthase beta subunit， glycosyltransferase family 2 protein， Rab7/RabG-family small GTPase， COP1-Interacting protein 7， cryptochrome 1a， ribosomal protein L16， transcription initiation factor TFIID subunit， 23.5 kDa heat-shock protein， POR， RIC1， NBS-LRR resistance protein， transcription factor， serine/threonine-protein kinase haspin， F-actin capping protein beta subunit， mitochondrial protein translocase family， maturase K， NADH dehydrogenase subunit F， chloroplast Tha 4-2， mitochondrial translational initiation factor， ATP binding protein， SNF-1 related kinase， heat shock protein 70 and lipase family protein. The identified proteins were found to de involved in diverse biological processes， covering energy pathway， glucose-metabolism， signal transduction， photoreaction， DNA and RNA synthesis and defense response. Proteome technique can be used to evaluation the selection effect of NIL.

CSTR: 32200.14.cjcb.2012.06.0008