温度和病原菌感染对大黄鱼热激蛋白90基因表达的影响

Abstract: Heat shock proteins (HSPs) existed in various organisms as a conserved protein family， and participated in many complex physiological processes. We cloned HSP90 gene and analysed the effect of temperature and pathogenic bacteria on its expression in Larimichthys crocea. The cloned HSP90 was 3 930 nt， including four exons and three introns. The open reading frame (ORF) was 2 178 nt encoding a polypeptide of 725 amino acids. Homology analysis showed that the deduced amino acid sequence of Larimichthys crocea HSP90 shared more than 90% identity with other fish. Under the different temperatures， HSP90 showed different expression patterns in the various tissues of Larimichthys crocea. HSP90 in heart， intestine and brain tissues had the strongest expression at 29 ºC， whereas HSP90 in skeletal muscle， spleen and liver tissues exhibited the strongest expression at 24 ºC. Larimichthys crocea was infected by intraperitoneal injection of pathogenic bacteria Pseudomonnas putida. HSP90 expression in gill， heart， spleen， intestine， kidney and brain tissues increased significantly after 48 h of injection. HSP90 expression in all examined nine tissues of the diseased fish was significantly higher than the control after 7 days of injection.

CSTR: 32200.14.cjcb.2012.06.0005