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The Expression of hnRNP A2/B1 and Regulation during the Apoptosis in Human Immortalized Epidermal HaCaT Cells Induced by Curcumin
Chen Lanying1, Yang Haibo1, Li Qifu2*
1Henan University of Urban Construction, Pingdingshan 467044, China; 2School of Life Sciences, Xiamen University, Xiamen 361005, China
Abstract: This paper explore the existence and distribution of hnRNP A2/B1 in nuclear matrix， and the colocalization relationship between hnRNP A2/B1 and the products of some apoptosis interrelated genes in the human immortalized epidermal HaCaT cells before and after curcumin treatment. It was confirmed by Western blot that hnRNP A2/B1 existed in the component of nuclear matrix protein of HaCaT cells and its expression was decreased by curcumin treatment. The co-localization between hnRNP A2/B1 and the products of apoptosis interrelated genes including Fas， p53 and Bax were observed by using laser scanning confocal microscopy. The recombinant protein was expressed and purified after building a prokaryotic expression vector pGEX-4T-2-hnRNP A2/B1， and GST pull-down assay confirmed that hnRNP A2/B1 directly interacted with Fas， p53 and Bax， respectively. The results of this study demonstrated that hnRNP A2/B1 was a nuclear matrix protein and locacted in the nuclear matrix， and the distribution of hnRNP A2/B1 and its relationship with associated gene products play important role during the apoptosis of HaCaT cells， which had great significance in in-depth understanding of regulatory mechanisms of nuclear matrix proteins in apoptosis.
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