The Prediction and Cytotoxicity Mechanism Analysis on the Functional Motif of the Membrance Pore-forming in rVvhA

Abstract: It was predicted that the area between the sites 141-335 of rVvhA amino acid sequence has the membrane pore-forming motif through the methods of bioinformatic. Using methods of geng-cloning， purification and renaturation， the rMpf proteins with purity of more than 95% was obtained. It can inhibit the growth of Hela cells and the effect was depended on doses， 0.8， 1.6， 2.4 μg/mL rMpf was used respectively for 8 hours， by transmission electron microscopy it was found that the cells show characteristic of apoptosis and the structure of mitochondrion was altered; intra-cellular ROS production was increased obvioulsy; mitochondrial membrane potential was lowered; mitochondrial permeability transition pore activity was increased as revealed by mPTP’s fluorescence inspection. The above data show that rMpf plays a role in inducing Hela cells’ damage， and leads to cell apoptosis via influencing cell membrane permeability.

CSTR: 32200.14.cjcb.2012.06.0002