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The Mechanism of MicroRNA-133b-mediated Inhibition of Human Rhabdomyosarcoma Cell Proliferation and Migration
Wang Lihua1#*, Chen Tongke2#, Yao Shasha1, Chen Xiaoyan1, Wang Jiao1
1School of Ophthalmology and Optometry, Wenzhou Medical College, Wenzhou 325027, China;2Laboratory Animal Center, Wenzhou Medical College, Wenzhou 325000, China
Abstract: The Real-time RT-PCR assay showed microRNA-133b was highly expressed in human muscle tissues but downregulated in rhabdomyosarcoma cell line RD and A204. MicroRNA-133b was transfected into RD and A204 cells by lipofectamine. The proliferation of RD and A204 cells was examined by MTS cell proliferation assay and the migration of RD and A204 cells was examined by Transwell migration assay. The results showed that microRNA-133b can inhibit the proliferation and migration of RD and A204 cells. The expression level of cell proliferation， migration and cell cycle related proteins was determined by Western blot. MicroRNA-133b downregulated the expression of LASP1， c-MET， phosphorylated-MET， phosphorylated-AKT， phosphorylated-ERK1/2， phosphorylated-Rb， CDK4 and CDK6 in RD and A204 cells. These results indicate that microRNA-133b inhibits proliferation and migration of rhabdomyosarcoma cells through down-regulation of LASP1， c-MET， phosphorylated- MET， phosphorylated-AKT， phosphorylated-ERK1/2， phosphorylated-Rb， CDK4 and CDK6.
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