Home > Browse Issues > Vol.30 No.2
Distribution of Human Bone Marrow-derived Stromal Cells Following Intravenous Injection into Nude Mice
Wei-Yan Zou1,3, Xiao-Cong Kuang1, Ying Wei1, Ming-Yi Zhu1, Ying-Hua Huang1, Guan-Mei Wen2*
1Department of Pathophysiology, Guangxi Medical University, Nanning 530021, China; 2Department of Pathophysiology, Guangzhou Medical College, Guangzhou 510182, China; 3Department of Histology and Embryology, Bengbu Medical
Abstract: human bone marrow-derived stromal cells (hBMSCs) were isolated by gradient centrifugation and were ex vivo-expanded using adhesion culture method. Morphology， phenotypes， cell growth dynamics， and osteogenic differentiation of hBMSCs were analyzed prior to intravenous injection into unconditioned nude mice. Culture-expanded hBMSCs were subsequently infused into the unconditioned recipients via tail vein injection. The donor-derived engraftment was qualified by reverse transcription polymerase chain reaction (RT-PCR) targeting human Alu gene at one week and four weeks postsystemic injection. The current results demonstrated that ex vivo-expanded hBMSCs phenotypically and morphologically appeared as CD34-CD45- spindle-shaped fibroblastic cells with the ability to give rise to osteogenic lineage in vitro. However analysis of growth dynamic of hBMSCs (P1-P5) showed no significant differences (P>0.05). Infused cells were present in multiple organs or tissues recovered from the recipients including lungs， liver， spleen， kidneys， skeletal muscle， heart， small intestines， and brain at 7 days and 4 weeks after cell transplantation. These data indicate that ex vivo-expanded hBMSCs distribute broadly following systemic infusion.
CN
EN