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Effect of Protein Kinase C (PKC) and Protein Kinase A (PKA) Signaling Pathways on the Expression of VEGF in Cultured Human Dental Follicle Cells
Chen Xuepeng1, Duan Yinzhong2, Qian Hong2, Jin Zuolin2*
1Department of Orthodontics, Stomatological Hospital Affiliated to Medical College of Zhejiang University, Hangzhou 310006, China; 2Department of Orthodontics, College of Stomatology, Fourth Military Medical University, Xi’an 710032,
Abstract: The aim of this paper is to study whether PKC and PKA signaling pathways are involved in regulation of expression of VEGF in cultured human dental follicle cells (HDFC). HDFC in good status were incubated with PMA (PKC activator)， PMA+Gö6983 (PKC non-specific inhibitor)， PMA+HBDDE (PKC-α and γ specific inhibitor)， PMA+LY333531 (PKC-β specific inhibitor)， dbcAMP (PKA activator)， dbcAMP+KT5720 (PKA inhibitor) for 2 h， respectively. Real-time PCR and Western blot were used to detect the gene and protein expression of VEGF in these groups respectively. The results showed that the gene and protein expression of VEGF in the group with PMA alone or the group with PMA+HBDDE were significantly higher than that of the control (P<0.05). There was no significant difference in the gene and protein expression of VEGF between the group with PMA+Gö6983 (or the group with PMA+LY333531) and the control (P>0.05). dbcAMP can up-regulate the gene and protein expression of VEGF in cultured HDFC， while this effect can be inhibited by KT5720. In conclusion， these findings suggest that the expression of VEGF in cultured HDFC can be regulated via PKC and PKA signaling pathways. In PKC signaling pathway， PKC-β may be the isoform that regulates VEGF expression in cultured HDFC.
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