Home > Browse Issues > Vol.34 No.5
Establishing A Leukemia-inducible Model with ES Cells
Liu Ling1, Liu Yanfeng1, Cheng Hui1, Jin Hui1, Zhang Quan’e1, Wang Zhengyu1,Yuan Weiping1, Gao Shaorong2, Xu Jing1, Cheng Tao1*
1State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China; 2National Institute of Biological
Abstract: Embryonic stem cells are pluripotent cell lines isolated from the inner cell mass of blastocyst that have the potential for multi-lineage differentiation. Transgenic animals are generated by introducing exogenous gene into ES cells. This approach has been used successfully for the analysis of gene function and regulation. Transfer of ES cells that contain virus-transduced transgene into blastocysts can also generate transgenic animals with germ-line transmission that stably express the transgene in mouse tissues. In this study， we confirmed that lentiviruses containing our construct can efficiently deliver hPML-RARa to murine ES cells with normal karyotypes. The infected ES cells were injected into blastocysts and then transplanted into pseudo-pregnant mice. Three chimeric mice carrying hPML-RARa were obtained with one confirmed as germ-line transmission competent. We then fed mice bearing the transgene with doxycycline in vivo and observed the expression of hPML-RARa in bone marrow cells after 3 days of Dox administration. It showed that the exogenous gene expression could be induced in vivo. Our results demonstrate that we have successfully established a leukemia-inducible transgenic mouse model with ES cells.
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