Home > Browse Issues > Vol.34 No.5
Drosophila Novel Alkaline Phosphatase Related Gene CG6236 Deletion and Phenotypic Analysis
Miao Haifeng1, Fang Kexin1, Huang Shuangshuang2, Jin Longjin2, Lin Xinhua3, Wu Yihui3*
1School of Optometry and Ophthalmology, Wenzhou Medical College, Wenzhou 325027, China; 2School of Medical Lab Science & School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China; 3State Key Laboratory of Biomembrane an
Abstract: Alkaline phosphatase (ALP) is present in all tissues through the entire body in human， and the abnormal ALP level is related to many diseases. However， the molecular mechanism of ALP during development remains unclear. Here， we report and analyze the function of gene CG6236， an important ALPlike gene， using Drosophila model system. We generated 3 mutant alleles for CG6236 using imprecise P-element excision method. We found that homo-mutant alleles were semi-lethal， and there were melanotic tumors in the abdomen of homozygous mutant larvaes and adult flies. Moreover， we generated pUAST-CG6236-V5 construct and its transgenic lines. The staining results showed that the protein of CG6236 was localized in the cytoplasm. Furthermore， by mosaic clone analysis and over-expression of CG6236 in wing discs， we showed that CG6236 is not required for Wingless and Hedgehog signaling. Taken together， this is the first time to generate CG6236 mutants and transgenic lines in Drosophila， and uncover the phenotype of a novel ALP related gene CG6236. Our findings provided evidence and laid foundation for further research to elucidate the physiological function and molecular mechanism of CG6236.
CN
EN