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Expression Increasing of mTOR Gene in Endometrium of Early Pregnant Mouse
Lan Zeng1, Jun-Lin He2, Yu-Bin Ding2, Xue-Mei Chen2, Ying-Xiong Wang2, Xue-Qing Liu1*
1Laboratory of the Reproductive Biology, 2Department of Genetics, School of Public Health, Chongqing Medical University, Chongqing 400016, China
Abstract: To explore the expression rule of mammalian target of rapamycin (mTOR) gene in endometria of early pregnant mouse， real-time fluorescence quantitative PCR (RT FQ-PCR)， in situ hybridization， immunohistochemistry and Western blotting were used to detect the mTOR mRNA and protein in the mouse endometria from unpregnant， pseudopregnant and pregnant d3， d4， d5， d6 and d7. Results showed that the mTOR expression in pregnant groups was higher than that of non-pregnant group (P<0.05)， and the mTOR expression increased significantly in the implantation window (P<0.05). By in situ hybridization and immunohistochemistry， mTOR expression of stroma cell in endometria was higher than that of uterine epithelium cell (P<0.05). The above results indicated that the expression rule of the mTOR in endometrial stromal cell may be one of the implantation mechanisms by regulating cell growth， proliferation and differentiation.
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