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The Effect of Hepatocyte Growth Factor Gene Transfection on the Apoptosis of Human Lymphoma Cell Line Raji Cells
Shen Rongrong1, Zheng Xiaojiao1, Cen Dong1,2*, Zhao Hang1, Hua Shixuan1, Pei Renzhi3, Lü Jianxin1, Tu Zhiguang4
1Zhejiang Provincial Yinzhou District Centre for Disease Control and Prevention, Ningbo 315100, China; 2Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou 325027, China; 3Department of
Abstract: To investigate hepatocyte growth factor (HGF) gene transfection and its inhibitory effect on the apoptosis of human lymphoma cell line Raji cells induced by VP-16， three cell lines (non-transfected Raji cells， empty vector transfected Raji cells and pVITRO2-mcs-HGF gene transfected Raji cells) were designed into normal groups and drug groups. Western blot was used to estimate the HGF protein level. CCK-8 assay was used to measure proliferated inhibition on Raji cells induced by VP-16. Quantitative and qualitative analyses on the apoptosis of Raji cells were performed through transmission electron microscopy， flow cytometry， acridine orange (AO) fluorescent staining and HE staining. The results showed that HGF protein expression was evaluated. CCK-8 assay revealed that VP-16 can inhibit the proliferation of Raji significantly at the concentration of 100 μg/mL. Typical morphologic changes of cell apoptosis were observed under transmission electronic microscope. Flow cytometry results showed that the apoptotic rates of drug groups were significantly higher than the control groups (P<0.01)， indicated that the apoptotic rate was enhanced after treated with VP-16. The apoptotic rates of HGF gene transfection groups were significantly lower than the non-transfected group (P<0.05) and empty vector transfected group (P<0.05)， indicated that HGF gene transfection can significantly protect Raji cells from apoptosis induced by VP-16. The results of acridine orange (AO) fluorescent staining and HE staining indicated that HGF can decrease the apoptotic rate of Raji cells induced by VP -16.
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