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The Morphological Observation and Time-dependent Changes Analysis of Erythroblasts during Differentiation in Mouse Fetal Livers
Zheng Yajuan, Chen Tao, Yang Zuli, Cao Lingling, Mao Qunquan, Xue Jianyou, Wu Yeqing, Zhang Shifu*
Proteomics & Molecular Enzymology Lab of College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
Abstract: Mouse fetal liver is an important early-stage blood-forming organ. During hematopoiesis， the morphology change of erythroblasts is significant. In view of the volume change of erythroblasts， the quantity of the diameter 8 μm to 14 μm erythroblasts from mouse E12.5-E17.5 fetal liver was measured by Count star cytometer. The erythroblasts from mouse E12.5-E17.5 fetal liver were divided into ten groups based on their morphology and hemoglobin expression. This study showed that with the development of fetal liver hematopoietic system， cell volume reduction， nuclear condensation， denucleation and hemoglobin up-regulation as well as time-dependent changes occurred during the terminal differentiation of mammalian erythroblasts. Quantitative immunocytochemical analysis of erythro-specific marker Ter119 and CD71 further confirmed that the differentiation capability of fetal liver erythroblasts was higher than that of adult bone marrow cells and peripheral blood cells. These data together provided theoretical support to investigate the mechanisms of erythroid differentiation and erythroleukemia.
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