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Efficient Induction of a U14-specific Anti-tumor Response by Dendritic Cells Sensitisized with ADM-U14 Antigen
Huang Yonghong1, Wang Hongmei1*, Zeng Xiaoping2, Cai Zhenyu1, Xu Fangyun1
1Department of Pathopysiology, College of Basic Medical Science, Nanchang University, Nanchang 330006, China; 2Department of Immunology, College of Basic Medical Science, Nanchang University, Nanchang 330006, China
Abstract: Studies have shown that tumor cells treated with chemotherapy drugs can effectively stimulate the immune response， which is dependent on the nature of the tumor cells and chemotherapy drugs. We reported bone marrow derived-DCs were sensitized with the culture supernatants from mice ADM-U14 after centrifugation. Then， the lymphocyte proliferation and the cytotoxic effects induced by the sensitized DCs were evaluated using MTT assays. We found the number of lymphocyte proliferation and the killing effects on mice U14 cells in the sensitized DCs group were much higher than those in the control group. Thus our results suggest mice ADM-U14 antigen can effectively sensitize DCs， and activate T lymphocytes proliferation and produce cytotoxic T lymphocytes (CTLs) to kill tumor cells in vitro.
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