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Analysis of Mitochondria Respiratory Function Associated with Motility of Sperm and Nucleus DNA Damage
Zheng Jiujia1,2, Lou Zhefeng1, Zheng Weihong3, Jin Jianyuan2, Ni Wuhua2, Li Ping1, Jin Longjin1*
1Zhejiang Provincial Key Laboratory for Medical Genetics, School of Life Sciences, School of Laboratory Medicine, Wenzhou Medical College, Wenzhou 325035,China; 2Reproductive Medicine Center, First Affiliated Hospital, Wenzhou Medica
Abstract: The aim of this study is to investigate the correlationship between mitochondrial respiratory function and sperm motility and nuclear DNA damage. Thirty-four semen samples were collected according to the WHO motility criteria. The mitochondria of sperm were extracted by differential centrifugation or density gradient centrifugation. Oxygen electrode units were used to measure the respiration rate of sperm mitochondria， and calculate mitochondrial state III respiration(ST III)， mitochondrial state IV respiration(ST IV)， P/O ratio， respiratory control rate(RCR) and the rate of oxidative phosphorylation(OPR). Sperm DNA damage was assessed by sperm chromatin dispersion(SCD). Our results showed that mitochondrial state III respiration significantly different among groups with different motility sperm(P<0.01)， the RCR and OPR in asthenospermia(AST) group were significantly lower than that of control group， decreased by 17.03%(P<0.05) and 40.74%(P<0.001)， respectively. Linear regression analysis indicated sperm DNA damage negative correlation to motility of sperm， STIII， OPR(r=-0.812， -0.788 and -0.696， respectively). In conclusion， mitochondria respiratory and oxidative phosphorylation might have close correlation with sperm motility; sperm function might be impaired when DNA was damaged.
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