The Expressing and Inducing Activity of Chimeric Neurturin in Rhesus Adipose Stem Cells

Abstract: In this study， we have shown that forced expressions of chimeric Nerve growth factor (NGF)/ Neurturin (NTN) in adult rhesus adipose mesenchymal stem cells (rASCs) facilitate dopaminergic neuron differentiation following adenovirus vector transduction in vitro. The repair potential of ASC-NTN in gene therapy in Rhesus model of Parkinson’s disease will be sought in the further work. In this study， rhesus ASCs were exposed to a recombinant chimeric NGF/NTN adenovirus which constructed and conserved in this lab expressing the NTN. The expression and location of chimeric NGF/NTN could be identified using RT-PCR， immunofluorescence staining and ELISA technology. rASCs were induced in the microenvironment combined with other chemical inducer. We detected the NTN transcripts in rASCs. Expression product could be secreted into the extracellular. The culture of dorsal root ganglia of chick embryo test had indicated that Neurturin secreted by rASCs had positive biological activity in nervous ramification forming. The ventral mesencephalic neurons of embryo rat survived with chimeric Neurturin in the culture. rASCs are able to differentiate into neuron like cells involving exogenous and endogenous factors. Our results demonstrated that rhesus adipose mesenchymal stem cells were able to specifically differentiate into neuron like cells by a mechanism involving NTN of the neuron developmental pathway when infected with Ad-NGF/NTN cultured in an appropriate exogenous chemical microenvironment. The operation of rASCs-NTN transplantation in Rhesus Parkinson’s disease model would be researched in the future based on this work.

CSTR: 32200.14.cjcb.2012.01.0004