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Comparative Proteomics Study of Chondrocytes Encapsulated in Alginate under Dynamic Compression
Ren Liling1*, Feng Xue2, Ma Dongyang3, Yu Zhanhai1, Chen Fulin4, Ding Yin2*
1School of Stomatology, Lanzhou University, Lanzhou 730000,China; 2Department of Dental Orthopaedics, School of Stomatology, the Fourth Military Medical University, Xi’an 710032, China; 3Department of Oral and Maxillofacia
Abstract: Mechanical stresses are known to play important role in articular cartilage functions in vivo. However， the molecular events of chondrocytes in respose to dynamic stress are still not well understood so far. In the present study， rabbit articular cartilage cells cultured in alginate beads were exposed to 0.2 MPa， 0.66Hz cyclic loadings for 4 hours. The differently expressed proteins involved in chondrocytes cultured in alginate beads under dynamic stress loading and unloading were identified by using comparative proteomics technology. 10 differently expressed proteins were found. In the 10 proteins， there were 6 up-regulated ones， 2 disappeared ones and 2 new appearance. Furthermore， by searching in protein and pertide databases， six meaningful proteins were identified， they were: prolyl 4-hydroxylase alpha I， pyruvate kinase， L-lactate dehydrogenase A， prolyl 4-hydroxylase subunit beta， destrin isoform and alpha enolase. Our research provided fundamental information on the differential expression of protein of chondrocytes cultured in alginate under dynamic stress. However， futher studies need to be done to better understand the molecular mechanisms of cartilage remodeling induced by dynamic stress loading.
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