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Effects of Glucocorticoid-induced Leucine Zipper (GILZ) on the Osteogenic Differentiation of Mouse Bone Marrow-derived Mesenchymal Stem Cells
He Linlin1,2, Lan Fei2, Xue Xiaoping1*
1Faculty of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China;2Institute of Molecular Medicine and Genetics, Georgia Health Sciences University, Augusta 30912, USA
Abstract: To investigate the effect of glucocorticoid-induced leucine zipper (GILZ) overexpression on mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) osteogenic differentiation， green fluorescent protein (GFP) and GILZ genes were delivered into BM-MSCs mediated by retrovirus and MSC-GFP and MSC-GILZ cells were prepared. MSC-GILZ cells were cultured in osteogenic medium (OS) using MSC-GFP cells as a control group. The results showed that GILZ significantly increased ALP expression and bone nodules formation. Real-time RT-PCR and Western blot results showed that GILZ significantly increased Runx2 and Osterix mRNA and protein expression. The results indicated that GILZ enhanced mouse BM-MSCs osteogenic differentiation partly through the increasing expression of Runx2 and Osterix which are the two key transcription factors of osteogenic differentiation.
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