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The Uptake Mechanism of Cationic Liposome/DNA Complex in Hela Cells
Ding Huiqin1,2, Cui Shaohui2, Wang Bing2, Hong Yang1, Zhang Shubiao2*
1College of Basic Medical Science, China Medical University, Shenyang 110001, China; 2Key Laboratory of State Ethnic Affairs Commission-Ministry of Education, College of Life Sciences, Dalian Nationalities University, Dalian 116600,
Abstract: The aim of this paper is to study the uptake mechanism for the cationic liposome/DNA complex by Hela cells. Eukaryotic cell membrane transport-related pathways were inhibited by inhibitors， and the gene with green fluorescent protein or luciferase was transfected into cells by cationic liposome. The transfection efficiency with different concentrations of inhibitors was detected by fluorescence microscope and low light level detector qualitatively or quantitatively. The MTT assay was used to detect the cell viability in order to evaluate the dependence on the pathways. The results suggested that when the cell viability remained at 60% or more， the fluorescence intensity significantly decreased and the efficiency of gene expression declined with the increase of the concentration of inhibition. This showed that the liposome/DNA complex was uptaken through the pathway which mediated by clathrin or caveolin and also through the phagocytosis that mediated by microtubule filaments.
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